Pyronin-Methyl Green: A Brilliant Double Stain for Cells and Bacteria

Methyl green-pyronin stain for the diagnosis of trachoma.

The clinical diagnosis of TRIC-agent infection of the conjunctiva is not difficult for ophthalmologists in endemic areas such as Egypt, but the similarity of the disease to other types of follicular conjunctivitis makes laboratory investigations especially important (Thygeson, I960; Duke-Elder, I965). These include the staining of conjunctival scrapings with Lugol's iodine or Giemsa stain for d...

Methyl Green-pyronin

Study of the stoichiometry of the DNA-methyl green reaction by dialysis, precipitation of stain-nucleic acid mixtures, and the staining of nuclei of known DNA content, indicate that the compound consists of one dye molecule per 10 P. The significance of this result was discussed in the preceding paper (1). Histone and lanthanum (and probably other multivalent cations (3)) compete with the dye f...

Methyl-green-pyronin as a differential nucleic acid stain for peripheral blood smears.

One-step staining of Neisseria gonorrhoeae in urethral discharge by methyl green-pyronin.

Methyl green-pyronin (MGP) was used in a one-step procedure to stain smears of urethral discharge from 169 men. Duplicate smears were stained by Gram's method and discharge was cultured for Neisseria gonorrhoeae. The organisms were isolated from 67 specimens and intracellular diplococci were seen in 74 smears after Gram staining and in 77 after staining by MGP. Furthermore, more extracellular a...

Modified Selenite Brilliant-green Medium

DAY, W. C., GOTTLIEB, S., AND PELCZAR, M. J. 1952 The biological degradatioin of lignin; The inability of Polyporous versicolor to metabolize sodium lignosulfonate. Appl. Microbiol., 1, 78-81. FOLIN, 0. 1929 Two revised copper methods for blood sugar determination. J. Biol. Chem., 82, 83-93. KAYE, S. 1949 The sterilizing action of gaseous ethylene oxide; The effect of ethylene oxide and related...